Bezmiâlem Science (Apr 2021)
Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae)
Abstract
Objective:Members of the Apiaceae family have many biological and pharmacological effects like anticancer, antidiabetic, antioxidant, anti-inflammatory, anti-hypertensive, anticoagulant, antimicrobial, and anticholinesterase. Also, some of them are consumed as vegetables, pickles, and spices. Carbonic anhydrase has a remarkable role in diseases like osteoporosis, glaucoma, edema, cancer, epilepsy, and obesity. The inhibition effects of methanol extracts and subextracts (n-hexane, dichloromethane, ethyl acetate, n-butanol, and aqueous residue) of Leiotulus dasyanthus and Ferulago pauciradiata roots on carbonic anhydrase I and II isoenzymes were investigated in this research.Methods:Leiotulus dasyanthus and Ferulago pauciradiata were collected in 2017 from Erzurum and in 2013 from Nevşehir (Turkey), respectively. The roots of L. dasyanthus and F. pauciradiata were macerated with methanol, dispersed with methanol: distilled water (1:9), and fractionated with n-hexane, dichloromethane, ethyl acetate, and n-butanol, in turn. The hCA I and II isoenzymes were isolated from erythrocytes of fresh human blood via Sepharose-4BL- Tyrosine-sulfanilamide affinity chromatography. The analysis of hCA I and II isoenzymes was actualized by the Verpoorte method.Results:The hCA I and II were influentially inhibited by the dichloromethane and ethyl acetate sub-extracts of F. pauciradiata root with IC50 values of 1.694 μg/mL towards hCA I and 1.677 μg/ mL towards hCA II, respectively. Whereas dichloromethane subextract and methanol extract of L. dasyanthus root inhibited hCA I and II isoenzymes with IC50 values of 1.828 μg/mL towards hCA I and 1.852 μg/mL towards hCA II, respectively.Conclusion:The hCA I and II were influentially inhibited by the dichloromethane and ethyl acetate sub-extracts of Ferulago pauciradiata root
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