Draft genome assemblies of the avian louse <i>Brueelia nebulosa</i> and its associates using long-read sequencing from an individual specimen

Abstract

AbstractSequencing high molecular weight (HMW) DNA with long-read and linked-read technologies has promoted a major increase in more complete genome sequences for nonmodel organisms. Sequencing approaches that rely on HMW DNA have been limited to larger organisms or pools of multiple individuals, but recent advances have allowed for sequencing from individuals of small-bodied organisms. Here, we use HMW DNA sequencing with PacBio long reads and TELL-Seq linked reads to assemble and annotate the genome from a single individual feather louse (Brueelia nebulosaSturnus vulgarisPediculus humanusBrueeliaSodalis