Nature Communications (Jan 2023)
Highly efficient and robust π-FISH rainbow for multiplexed in situ detection of diverse biomolecules
- Yingfeng Tao,
- Xiaoliu Zhou,
- Leqiang Sun,
- Da Lin,
- Huaiyuan Cai,
- Xi Chen,
- Wei Zhou,
- Bing Yang,
- Zhe Hu,
- Jing Yu,
- Jing Zhang,
- Xiaoqing Yang,
- Fang Yang,
- Bang Shen,
- Wenbao Qi,
- Zhenfang Fu,
- Jinxia Dai,
- Gang Cao
Affiliations
- Yingfeng Tao
- State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Xiaoliu Zhou
- State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Leqiang Sun
- State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Da Lin
- State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Huaiyuan Cai
- State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Xi Chen
- State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Wei Zhou
- State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Bing Yang
- State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Zhe Hu
- State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Jing Yu
- Department of Blood Transfusion, Wuhan hospital of Traditional Chinese and Western Medicine, Huazhong University of Science and Technology
- Jing Zhang
- Department of the 1st Thoracic Medical Oncology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology
- Xiaoqing Yang
- Hospital of Huazhong Agricultural University
- Fang Yang
- National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University
- Bang Shen
- State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Wenbao Qi
- College of Veterinary Medicine, South China Agricultural University
- Zhenfang Fu
- Departments of Pathology, College of Veterinary Medicine, University of Georgia
- Jinxia Dai
- State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University
- Gang Cao
- State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University
- DOI
- https://doi.org/10.1038/s41467-023-36137-4
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 15
Abstract
Fluorescence in situ hybridization (FISH) methods with high sensitivity are needed. Here the authors develop multiplex πFISH rainbow to detect a range of biomolecules; they also combine this with the hybridization chain reaction to develop πFISH+ technology for short nucleic acid fragment detection.
