A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion

Peng Chen; Xun Chen; R. Glenn Hepfer; Brooke J. Damon; Changcheng Shi; Jenny J. Yao; Matthew C. Coombs; Michael J. Kern; Tong Ye; Hai Yao

doi:10.1038/s41467-021-22221-0

Nature Communications (Mar 2021)

A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion

Peng Chen,
Xun Chen,
R. Glenn Hepfer,
Brooke J. Damon,
Changcheng Shi,
Jenny J. Yao,
Matthew C. Coombs,
Michael J. Kern,
Tong Ye,
Hai Yao

Affiliations

Peng Chen: Clemson-MUSC Joint Bioengineering Program, Department of Bioengineering, Clemson University
Xun Chen: Clemson-MUSC Joint Bioengineering Program, Department of Bioengineering, Clemson University
R. Glenn Hepfer: Clemson-MUSC Joint Bioengineering Program, Department of Bioengineering, Clemson University
Brooke J. Damon: Clemson-MUSC Joint Bioengineering Program, Department of Bioengineering, Clemson University
Changcheng Shi: Clemson-MUSC Joint Bioengineering Program, Department of Bioengineering, Clemson University
Jenny J. Yao: Department of Physics, Harvard University
Matthew C. Coombs: Clemson-MUSC Joint Bioengineering Program, Department of Bioengineering, Clemson University
Michael J. Kern: Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina
Tong Ye: Clemson-MUSC Joint Bioengineering Program, Department of Bioengineering, Clemson University
Hai Yao: Clemson-MUSC Joint Bioengineering Program, Department of Bioengineering, Clemson University

DOI: https://doi.org/10.1038/s41467-021-22221-0
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 17

Abstract

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It is challenging to quantify anisotropic diffusion in biological systems. Here the authors report light-sheet imaging-based Fourier transform fluorescence recovery after photobleaching (LiFT-FRAP) to noninvasively determine 3D diffusion tensors of various biomolecules at physiological diffusivity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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