Nature Communications (Oct 2017)

Multi-wavelength anomalous diffraction de novo phasing using a two-colour X-ray free-electron laser with wide tunability

  • Alexander Gorel,
  • Koji Motomura,
  • Hironobu Fukuzawa,
  • R. Bruce Doak,
  • Marie Luise Grünbein,
  • Mario Hilpert,
  • Ichiro Inoue,
  • Marco Kloos,
  • Gabriela Kovácsová,
  • Eriko Nango,
  • Karol Nass,
  • Christopher M. Roome,
  • Robert L. Shoeman,
  • Rie Tanaka,
  • Kensuke Tono,
  • Yasumasa Joti,
  • Makina Yabashi,
  • So Iwata,
  • Lutz Foucar,
  • Kiyoshi Ueda,
  • Thomas R. M. Barends,
  • Ilme Schlichting

DOI
https://doi.org/10.1038/s41467-017-00754-7
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 8

Abstract

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X-ray free-electron lasers produce bright femtosecond X-ray pulses. Here, the authors use a two-colour X-ray free-electron laser beam for simultaneous two-wavelength data collection and show that protein structures can be determined with multiple wavelength anomalous dispersion phasing, which is important for difficult-to-phase projects.