Nature Communications (Sep 2020)

Ultra-sensitive and rapid detection of nucleic acids and microorganisms in body fluids using single-molecule tethering

  • Wen-Chih Cheng,
  • Troy Horn,
  • Maya Zayats,
  • Georges Rizk,
  • Samuel Major,
  • Hongying Zhu,
  • Joseph Russell,
  • Zhiguang Xu,
  • Richard E. Rothman,
  • Alfredo Celedon

DOI
https://doi.org/10.1038/s41467-020-18574-7
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 9

Abstract

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Culture-based diagnostic methods for microorganism detection are time-consuming but still the gold standard for conditions such as sepsis. Here the authors present an amplification and purification-free method to detect microorganisms in bodily fluids with high sensitivity: Single MOLecule Tethering (SMOLT).