Frontiers in Veterinary Science (Jan 2020)
Prevalence and Genetic Characterization of Cryptosporidium, Giardia and Enterocytozoon in Chickens From Ezhou, Hubei, China
- Shengkui Cao,
- Shengkui Cao,
- Shengkui Cao,
- Shengkui Cao,
- Shengkui Cao,
- Meng Xu,
- Meng Xu,
- Meng Xu,
- Meng Xu,
- Meng Xu,
- Yanyan Jiang,
- Yanyan Jiang,
- Yanyan Jiang,
- Yanyan Jiang,
- Yanyan Jiang,
- Hua Liu,
- Hua Liu,
- Hua Liu,
- Hua Liu,
- Hua Liu,
- Zhongying Yuan,
- Zhongying Yuan,
- Zhongying Yuan,
- Zhongying Yuan,
- Zhongying Yuan,
- Lei Sun,
- Lei Sun,
- Lei Sun,
- Lei Sun,
- Lei Sun,
- Jianping Cao,
- Jianping Cao,
- Jianping Cao,
- Jianping Cao,
- Jianping Cao,
- Yujuan Shen,
- Yujuan Shen,
- Yujuan Shen,
- Yujuan Shen,
- Yujuan Shen
Affiliations
- Shengkui Cao
- National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China
- Shengkui Cao
- Chinese Center for Tropical Diseases Research, Shanghai, China
- Shengkui Cao
- Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, China
- Shengkui Cao
- WHO Collaborating Centre for Tropical Diseases, Shanghai, China
- Shengkui Cao
- National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China
- Meng Xu
- National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China
- Meng Xu
- Chinese Center for Tropical Diseases Research, Shanghai, China
- Meng Xu
- Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, China
- Meng Xu
- WHO Collaborating Centre for Tropical Diseases, Shanghai, China
- Meng Xu
- National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China
- Yanyan Jiang
- National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China
- Yanyan Jiang
- Chinese Center for Tropical Diseases Research, Shanghai, China
- Yanyan Jiang
- Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, China
- Yanyan Jiang
- WHO Collaborating Centre for Tropical Diseases, Shanghai, China
- Yanyan Jiang
- National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China
- Hua Liu
- National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China
- Hua Liu
- Chinese Center for Tropical Diseases Research, Shanghai, China
- Hua Liu
- Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, China
- Hua Liu
- WHO Collaborating Centre for Tropical Diseases, Shanghai, China
- Hua Liu
- National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China
- Zhongying Yuan
- National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China
- Zhongying Yuan
- Chinese Center for Tropical Diseases Research, Shanghai, China
- Zhongying Yuan
- Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, China
- Zhongying Yuan
- WHO Collaborating Centre for Tropical Diseases, Shanghai, China
- Zhongying Yuan
- National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China
- Lei Sun
- National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China
- Lei Sun
- Chinese Center for Tropical Diseases Research, Shanghai, China
- Lei Sun
- Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, China
- Lei Sun
- WHO Collaborating Centre for Tropical Diseases, Shanghai, China
- Lei Sun
- National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China
- Jianping Cao
- National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China
- Jianping Cao
- Chinese Center for Tropical Diseases Research, Shanghai, China
- Jianping Cao
- Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, China
- Jianping Cao
- WHO Collaborating Centre for Tropical Diseases, Shanghai, China
- Jianping Cao
- National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China
- Yujuan Shen
- National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China
- Yujuan Shen
- Chinese Center for Tropical Diseases Research, Shanghai, China
- Yujuan Shen
- Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, China
- Yujuan Shen
- WHO Collaborating Centre for Tropical Diseases, Shanghai, China
- Yujuan Shen
- National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, China
- DOI
- https://doi.org/10.3389/fvets.2020.00030
- Journal volume & issue
-
Vol. 7
Abstract
Cryptosporidium spp., Giardia spp. and microsporidia are important intestinal protozoa responsible for diarrhea in humans and other mammals. China is a major chicken-raising country, and studies on these protozoa in chickens have important public health significance. Here, we investigated the prevalence and genetic characterization of these parasites in chickens from Ezhou City, Hubei Province, China. In total, 206 stool specimens were collected from chickens in four villages of Ezhou between July 2014 and February 2015. Genomic DNA of each specimen was tested by nested PCR based on the Cryptosporidium small subunit rRNA gene, the Giardia intestinalis triose phosphate isomerase gene, and the internal transcribed spacer of the Enterocytozoon bieneusi rRNA gene, respectively. The public health significance of G. intestinalis and E. bieneusi identified in our study was evaluated via phylogenetic analysis. The infection rates were determined to be 2.43% (5/206), 8.25% (17/206), and 1.94% (4/206) for Cryptosporidium, G. intestinalis, and E. bieneusi, respectively. One sample showed coinfection with G. intestinalis and E. bieneusi. Meanwhile, sequence analysis of the PCR-positive samples showed that the Cryptosporidium was C. baileyi, G. intestinalis was assemblage C, and E. bieneusi was genotype D and novel genotype EZ0008. This is the first report of zoonotic G. intestinalis assemblage C in chickens in the world, and the first report of zoonotic E. bieneusi genotype D in chickens in China. These findings indicate new transmission dynamics and molecular epizootiology.
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