Patologìâ (Apr 2018)
Changes in the expression of mRNA TLR2 and 4 type, nuclear factor kВ and pro-inflammatory cytokines IL-1β and IL-17A in the epithelium of the oral cavity in children with special needs
Abstract
Abstract Objective: to assess the relative level of mRNA of proinflammatory cytokines IL-1β and IL-17A, toll-like receptors 2 and 4 types and the transcription factor NF-KB in the epithelium of the oral cavity in children with diseases of the central nervous system, blood system, respiratory organs, and in children with mental disorders. Object and methods: the molecular-genetic study by polymerase chain reaction with reverse transcription real-time (RT-PCR) mRNA expression of the genes TLR2, TLR4, NF-KB, IL-1β and IL-17A in the epithelium of the oral cavity in 93 children with disabilities and 25 children with chronic catarrhal gingivitis without associated pathology in age from 12 to 15 years. Results: In children with special needs there is the rise of transcription activity of membrane TLR2 and TLR4 in all study groups except the group of children with mental disorders. Activation of pattern recognition receptors naturally causes transcriptional induction of the gene NF-KB and regulated by it the proinflammatory cytokines IL-1β and IL-17A by the buccal epithelium: expression levels of mRNA of IL-1β and IL-17A in children with central nervous system diseases were increased by 8.9 and 7.7 times (P < 0.05), respectively, in children with mental diseases the level of IL-17A was increased by 2.2 times (P < 0.05) in comparison with the control, in children with respiratory diseases and diseases of the blood system the expression of IL-1β was increased by 6.5 and 2.9 times (P < 0.05), respectively, IL-17A – 3.8 and 3 times (P < 0.05) as compared to control. Conclusions: discovered in the work transcriptional induction of the genes TLR2 and TLR4 and subsequent activation of Nf-kB and production of proinflammatory cytokines IL-1β and IL-17A d is one of the mechanisms that explains the more severe course of periodontitis in children with disabilities as compared to patients with periodontitis and without comorbidities.
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