Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

Haiyun Pei; Hsu-Yuan Fu; Hiroyuki Hirai; Dong Seong Cho; Timothy D. O'Brien; James Dutton; Catherine M. Verfaillie; Wei-Shou Hu

doi:10.1016/j.scr.2017.04.010

Stem Cell Research (May 2017)

Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

Haiyun Pei,
Hsu-Yuan Fu,
Hiroyuki Hirai,
Dong Seong Cho,
Timothy D. O'Brien,
James Dutton,
Catherine M. Verfaillie,
Wei-Shou Hu

Affiliations

Haiyun Pei: Department of Chemical Engineering and Materials Science, Minneapolis, MN, USA
Hsu-Yuan Fu: Department of Chemical Engineering and Materials Science, Minneapolis, MN, USA
Hiroyuki Hirai: Department of Cardiac Surgery, University of Michigan, Ann Arbor, MI, USA
Dong Seong Cho: Department of Chemical Engineering and Materials Science, Minneapolis, MN, USA
Timothy D. O'Brien: Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA
James Dutton: Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA
Catherine M. Verfaillie: Department of Development and Regeneration, Katholieke Universiteit Leuven, Leuven, Belgium
Wei-Shou Hu: Department of Chemical Engineering and Materials Science, Minneapolis, MN, USA

DOI: https://doi.org/10.1016/j.scr.2017.04.010
Journal volume & issue: Vol. 21, no. C
pp. 132 – 136

Abstract

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We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M3O, Sox2, Klf4, and n-Myc) using a lentiviral vector. The cell line possess all the characteristics of an induced pluripotent stem cell (iPSC) line. Given that Chinese hamster ovary (CHO) cells are the predominant host cells used for therapeutic protein production and no pluripotent stem cell line or other normal cell line has been isolated from Chinese hamster, this iPSC line may serve as a useful tool for research using CHO cells or even be used for deriving new cell lines.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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