White Light Diffraction Phase Microscopy in Imaging of Breast and Colon Tissues
Adriana Smarandache,
Ruxandra A. Pirvulescu,
Ionut-Relu Andrei,
Andra Dinache,
Mihaela Oana Romanitan,
Daniel Constantin Branisteanu,
Mihail Zemba,
Nicoleta Anton,
Mihail-Lucian Pascu,
Viorel Nastasa
Affiliations
Adriana Smarandache
Laser Department, National Institute for Laser, Plasma and Radiation Physics, 077125 Magurele, Romania
Ruxandra A. Pirvulescu
Department of Ophthalmology, University of Medicine and Pharmacy “Carol Davila”, 020022 Bucharest, Romania
Ionut-Relu Andrei
Laser Department, National Institute for Laser, Plasma and Radiation Physics, 077125 Magurele, Romania
Andra Dinache
Laser Department, National Institute for Laser, Plasma and Radiation Physics, 077125 Magurele, Romania
Mihaela Oana Romanitan
Department for Emergency Internal Medicine and Neurology, Stockholm South General Hospital, 11883 Stockholm, Sweden
Daniel Constantin Branisteanu
Department of Ophthalmology, University of Medicine and Pharmacy “Grigore T Popa”, 700115 Iasi, Romania
Mihail Zemba
Department of Ophthalmology, University of Medicine and Pharmacy “Carol Davila”, 020022 Bucharest, Romania
Nicoleta Anton
Department of Ophthalmology, University of Medicine and Pharmacy “Grigore T Popa”, 700115 Iasi, Romania
Mihail-Lucian Pascu
Laser Department, National Institute for Laser, Plasma and Radiation Physics, 077125 Magurele, Romania
Viorel Nastasa
Extreme Light Infrastructure-Nuclear Physics ELI-NP, “Horia Hulubei” National Institute for Physics and Nuclear Engineering IFIN-HH, 077125 Magurele, Romania
This paper reports results obtained using white light diffraction phase microscopy (wDPM) on captured images of breast and colon tissue samples, marking a contribution to the advancement in biomedical imaging. Unlike conventional brightfield microscopy, wDPM offers the capability to capture intricate details of biological specimens with enhanced clarity and precision. It combines high resolution, enhanced contrast, and quantitative capabilities with non-invasive, label-free imaging. These features make it a useful tool for tissue imaging, providing detailed and accurate insights into tissue structure and dynamics without compromising the integrity of the samples. Our findings underscore the potential of quantitative phase imaging in histopathology, in the context of automating the process of tissue analysis and diagnosis. Of particular note are the insights gained from the reconstructed phase images, which provide physical data regarding peripheral glandular cell membranes. These observations serve to focus attention on pathologies involving the basal membrane, such as early invasive carcinoma. Through our analysis, we aim to contribute to catalyzing further advancements in tissue (breast and colon) imaging.
