Scientific Reports (Jun 2018)

Methodology for Y Chromosome Capture: A complete genome sequence of  Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads

  • M. J. Alvarez-Cubero,
  • O. Santiago,
  • C. Martínez-Labarga,
  • B. Martínez-García,
  • R. Marrero- Díaz,
  • A. Rubio-Roldan,
  • A. M. Pérez-Gutiérrez,
  • P. Carmona-Saez,
  • J. A. Lorente,
  • L. J. Martinez-Gonzalez

DOI
https://doi.org/10.1038/s41598-018-27819-x
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 13

Abstract

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Abstract This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin–biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences.