Identification and application of a strong bidirectional acmN2p promoter from actinomycin D-producing streptomycetes

Abstract

Natural product biosynthesis is controlled at multiple levels. Characterization of naturally occurring promoters has facilitated the study of the synthetic biology of natural products. Herein, we report the discovery of two high-yield actinomycin D (ActD)-producing streptomycetes and the identification of a strong bidirectional acmN2p promoter from the ActD gene clusters and its application in heterologous expression of three core genes involved in the bacterial alkaloid bohemamine biosynthesis, providing a good example for identification of new promoters for synthetic biological applications.

Keywords

• Actinomycin D
• Bidirectional promoter
• Bohemamine
• Streptomyces