Expression of a Cytochrome P450 Gene from Bermuda Grass <i>Cynodon dactylon</i> in Soybean Confers Tolerance to Multiple Herbicides
Ting Zheng,
Xiaoxing Yu,
Yongzheng Sun,
Qing Zhang,
Xianwen Zhang,
Mengzhen Tang,
Chaoyang Lin,
Zhicheng Shen
Affiliations
Ting Zheng
State Key Laboratory of Rice Biology, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310000, China
Xiaoxing Yu
State Key Laboratory of Rice Biology, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310000, China
Yongzheng Sun
State Key Laboratory of Rice Biology, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310000, China
Qing Zhang
Hangzhou Ruifeng Biosciences Co., Ltd., 1500 Wenyi Road, Building 1, Room 103, Hangzhou 310000, China
Xianwen Zhang
Agricultural Experiment Station, Zhejiang University, Hangzhou 310000, China
Mengzhen Tang
Hangzhou Ruifeng Biosciences Co., Ltd., 1500 Wenyi Road, Building 1, Room 103, Hangzhou 310000, China
Chaoyang Lin
State Key Laboratory of Rice Biology, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310000, China
Zhicheng Shen
State Key Laboratory of Rice Biology, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310000, China
Bermuda grass (Cynodon dactylon) is notoriously difficult to control with some commonly used herbicides. We cloned a cytochrome P450 gene from Bermuda grass, named P450-N-Z1, which was found to confer tolerance to multiple herbicides in transgenic Arabidopsis. These herbicides include: (1) acetolactate synthase (ALS) inhibitor herbicides nicosulfuron and penoxsulam; (2) p-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicide mesotrione; (3) synthetic auxin herbicide dicamba; (4) photosynthesis inhibitor bentazon. We further generated transgenic soybean plants expressing P450-N-Z1, and found that these transgenic soybean plants gained robust tolerance to nicosulfuron, flazasulfuron, and 2,4-dichlorophenoxyacetic acid (2,4-D) in greenhouse assays. A field trial demonstrated that transgenic soybean is tolerant to flazasulfuron and 2,4-D at 4-fold and 2-fold the recommended rates, respectively. Furthermore, we also demonstrated that flazasulfuron and dicamba are much more rapidly degraded in vivo in the transgenic soybean than in non-transgenic soybean. Therefore, P450-N-Z1 may be utilized for engineering transgenic crops for herbicide tolerance.
