Artificially sporulated Escherichia coli cells as a robust cell factory for interfacial biocatalysis

Abstract

Whole-cell biocatalysis is of interest for the efficient production of a range of products but cell stability can be an issue. Here, the authors turn recombinant enzyme-producing E. coli into artificial spores to add protection from UV radiation, heating, and organic solvents by coating the cells with polydopamine to create robust cell factories.