PLoS ONE (Jan 2016)

Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model.

  • Shuya Yano,
  • Kiyoto Takehara,
  • Shinji Miwa,
  • Hiroyuki Kishimoto,
  • Yukihiko Hiroshima,
  • Takashi Murakami,
  • Yasuo Urata,
  • Shunsuke Kagawa,
  • Michael Bouvet,
  • Toshiyoshi Fujiwara,
  • Robert M Hoffman

DOI
https://doi.org/10.1371/journal.pone.0148760
Journal volume & issue
Vol. 11, no. 2
p. e0148760

Abstract

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Fluorescence-guided surgery (FGS) of cancer is an area of intense development. In the present report, we demonstrate that the telomerase-dependent green fluorescent protein (GFP)-containing adenovirus OBP-401 could label colon-cancer liver metastasis in situ in an orthotopic mouse model enabling successful FGS. OBP-401-GFP-labeled liver metastasis resulted in complete resection with FGS, in contrast, conventional bright-light surgery (BLS) did not result in complete resection of the metastasis. OBP-401-FGS reduced the recurrence rate and prolonged over-all survival compared with BLS. In conclusion, adenovirus OBP-401 is a powerful tool to label liver metastasis in situ with GFP which enables its complete resection, not possible with conventional BLS.