Fast and Noninvasive Hair Test for Preliminary Diagnosis of Mood Disorders
Magdalena Świądro-Piętoń,
Kai A. Morawiec,
Anna Wójtowicz,
Sara Świądro,
Rafał Kurczab,
Dominika Dudek,
Renata Wietecha-Posłuszny
Affiliations
Magdalena Świądro-Piętoń
Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 2, Gronostajowa St., 30-387 Kraków, Poland
Kai A. Morawiec
Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 2, Gronostajowa St., 30-387 Kraków, Poland
Anna Wójtowicz
Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 2, Gronostajowa St., 30-387 Kraków, Poland
Sara Świądro
Faculty of Mathematical and Natural Science, Department of Chemistry, University of Applied Sciences in Tarnow, 8 Mickiewicza St, 33-100 Tarnów, Poland
Rafał Kurczab
Faculty of Mathematical and Natural Science, Department of Chemistry, University of Applied Sciences in Tarnow, 8 Mickiewicza St, 33-100 Tarnów, Poland
Dominika Dudek
Department of Adult Psychiatry, Jagiellonian University Medical College, 21a, Mikołaja Kopernika St., 31-501 Kraków, Poland
Renata Wietecha-Posłuszny
Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 2, Gronostajowa St., 30-387 Kraków, Poland
The main objective of this study was to develop a test for the fast and noninvasive prediagnosis of mood disorders based on the noninvasive analysis of hair samples. The database included 75 control subjects (who were not diagnosed with depression) and 40 patients diagnosed with mood disorders such as depression or bipolar disorder. Both women and men, aged 18–65 years, participated in the research. After taking the hair samples, they were washed (methanol–water–methanol by shaking in a centrifuge for two min) and air-dried in a fume hood. Each hair collection was analyzed using Fourier transform infrared spectroscopy attenuated total reflection (ATR-FTIR) spectroscopy. Subsequently, the results obtained were analyzed based on chemometric methods: hierarchical cluster analysis (HCA) and principal component analysis (PCA). As a results of the research conducted, potential differences were noticed. There was a visible change in the spectra intensity at around 2800–3100 cm−1 and smaller differences around 1460 cm−1; the bands can be assigned to protein vibrations. However, these are preliminary studies that provide a good basis for the development of a test for the initial diagnosis of mood disorders.
