Sperm selection of cryopreserved milt of catfish (Rhamdia quelen) by density gradient centrifugation with AllGrad® 90%
Maritza Pérez-Atehortúa,
Andrea Galuppo,
Rômulo Batista Rodrigues,
Nathalia dos Santos Teixeira,
Thaiza Rodrigues de Freitas,
Thales de Souza França,
Lis Santos Marques,
Itamar Cossina Gomes,
Danilo Pedro Streit, Jr.
Affiliations
Maritza Pérez-Atehortúa
Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil
Andrea Galuppo
Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil
Rômulo Batista Rodrigues
Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil
Nathalia dos Santos Teixeira
Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil
Thaiza Rodrigues de Freitas
Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil
Thales de Souza França
Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil
Lis Santos Marques
Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil
Itamar Cossina Gomes
Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil
Danilo Pedro Streit, Jr.
Corresponding author.; Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil
Density gradient centrifugation is a technique used to wash or separate samples of cryopreserved milt, mainly in humans and bovines allowing, for example, reducing the concentration of cryoprotectants or choosing the best portion of sperm. The proposed method seeks to reduce the presence of cryoprotectant in the cryopreserved milt of the Rhamdia qhelen and to obtain a fraction of better quality sperm. Gradient centrifugation was formed from 90% AllGrad® and different centrifugation times and forces were compared. The separated sperm presented a low increase in motility and decreased head damage and presence of gout, however, it was better compared to the non-separated samples. The speed of 1000 × g for 10 min, 4 °C, allowed 22.25 ± 4.64% of normal spermatozoa, that is, 9.25% more than the non-centrifuged milt (p = 0.0013). • The centrifugation method allows a fraction of spermatozoa morphologically less affected by cryopreservation. • Density gradient centrifugation with AllGrad® 90% is proposed as a tool of easy adaptation and application for the separation of cryopreserved sperm of R. quelen. • Density gradient centrifugation method at 1000 × g for 10 min allows obtaining a better fraction of normal sperm.
