Determination of Poly(3-hydroxybutyrate) Content in Cyanobacterium Synechocystis sp. PCC 6803 Using Acid Hydrolysis Followed by High-performance Liquid Chromatography
Janine Kaewbai-ngam,
Aran Incharoensakdi,
Tanakarn Monshupanee
Affiliations
Janine Kaewbai-ngam
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandProgram in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
Aran Incharoensakdi
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandAcademy of Science, Royal Society of Thailand, Bangkok 10300, Thailand
Tanakarn Monshupanee
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandProgram in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
Various photoautotrophic cyanobacteria accumulate intracellular poly(3-hydroxybutyrate) (PHB) granules. This protocol can be used for determining the PHB contents of the cells as % PHB weight per dry cell weight using acid hydrolysis followed by high-performance liquid chromatography (HPLC). This HPLC analysis is rapid, with a running time of approximately 5 min per sample. The technique can accurately determine PHB concentrations in the range of 2–1,000 μg/mL PHB. However, this technique is not applicable for determining the contents of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in cyanobacteria.
