Spectrophotometric Estimation of Ganciclovir in Pharmaceutical Formulations using Alizarin Sulfonic Acid Sodium Salt Reagent

Abstract

الملص ABSTRACT A simple and rapid spectrophotometric method has been developed for the determination of antiviral drug (Ganciclovir) in a bulk sample and in its dosage forms. The method depends on the ion-pair formation reaction of the drug with alizarin sulfonic acid sodium salt reagent in aqueous solution to form a pinkish - red color product showing maximum absorbance at 525 nm. Beer's law is obeyed in the concentration range 2 – 100 µg.ml-1 with average recovery (accuracy) 99.80% and precision (RSD) is less than 1.0%. The molar absorptivity is 4.59x103 l.mol-1.cm-1 with LOD 0.17 µg.ml-1 and LOQ 0.56 µg.ml-1. The method is successfully employed for the determination of Ganciclovir in pharmaceutical formulations as an injection and capsule and the results are compatible with both certified values of pharmaceutical formulations and the standard addition method. Keywords: ganciclovir; spectrophotometric; alizarin sulfonic acid sodium salt.

Keywords

• spectrophotometric estimation
• pharmaceutical formulations
• alizarin sulfonic