INFLUENCE OF MEDICAL LEECH’S EXOGENIC BIOLOGICALLY ACTIVE SUBSTANCES ON BIOLOGICAL CHARACTERISTICS OF ESСHERICHIA COLI 3912/41

Abstract

Purpose. To ascertain the effect of exogenous bioactive substances of cultural water, which contained the medicinal leeches on biological properties of Escherichia coli 3912/41. Methods. There were prepared the meat-peptone agar (MPA) with various concentrations of the cultural water, which contained the medical leech: 0, 0.1, 1, 5, 10, 15%. On the surface of the MPA with appropriate concentration of cultural water (experiment) and without (control) there were added daily working suspension culture of bacteria E. coli 3912/41 cells at the concentration of 850 CFU/ml. The cultural and morphological properties of E. coli were investigated. Change of the antigenic properties of the reaction was studied in slide agglutination with serum diagnostic polyvalent dry to OKA-antigens of Escherichia for agglutination reaction. Results. There were found the cultural properties oppression of E. coli, characterized by the decrease in the colonies from 1% water content of the culture, which contained medicinal leeches in MPA. At microscopic analysis there were detected the bacteria reduction in size to coccus-like forms of bacteria. The antigenic properties of species E. coli infringements with increasing concentration of the cultural water in MPA were found. Conclusions. The cultural water, which contained the medical leech, depending on concentration in MPA inhibits cultural and antigenic properties of E. coli. The results indicate the perspective of using cultural water, which contained medicinal leeches as a source of biologically active substances with a bacteriostatic effect on opportunistic bacteria.

Keywords

• esсherichia coli
• біологічно активні речовини
• медична п’явка
• культуральна вода
• бактеріостатичний ефект