Scientific Reports (May 2017)

High throughput single cell counting in droplet-based microfluidics

  • Heng Lu,
  • Ouriel Caen,
  • Jeremy Vrignon,
  • Eleonora Zonta,
  • Zakaria El Harrak,
  • Philippe Nizard,
  • Jean-Christophe Baret,
  • Valérie Taly

DOI
https://doi.org/10.1038/s41598-017-01454-4
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 9

Abstract

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Abstract Droplet-based microfluidics is extensively and increasingly used for high-throughput single-cell studies. However, the accuracy of the cell counting method directly impacts the robustness of such studies. We describe here a simple and precise method to accurately count a large number of adherent and non-adherent human cells as well as bacteria. Our microfluidic hemocytometer provides statistically relevant data on large populations of cells at a high-throughput, used to characterize cell encapsulation and cell viability during incubation in droplets.