Bioformulation of mineral solubilizing microbes as novel microbial consortium for the growth promotion of wheat (Triticum aestivum) under the controlled and natural conditions
Rubee Devi,
Tanvir Kaur,
Rajeshwari Negi,
Divjot Kour,
Sanjeev Kumar,
Ashok Yadav,
Sangram Singh,
Kundan Kumar Chaubey,
Ashutosh Kumar Rai,
Sheikh Shreaz,
Ajar Nath Yadav
Affiliations
Rubee Devi
Department of Genetics, Plant Breeding and Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour,173101, Himachal Pradesh, India
Tanvir Kaur
Department of Genetics, Plant Breeding and Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour,173101, Himachal Pradesh, India
Rajeshwari Negi
Department of Genetics, Plant Breeding and Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour,173101, Himachal Pradesh, India
Divjot Kour
Department of Microbiology, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmour, 173101, Himachal Pradesh, India
Sanjeev Kumar
Department of Genetics and Plant Breeding, Faculty of Agricultural Science, GLA university, Mathura, Uttar Pradesh, India
Ashok Yadav
Department of Botany, Institute of Science, Banaras Hindu University, Varanasi, Uttar Pradesh, India
Sangram Singh
Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh, India
Kundan Kumar Chaubey
Division of Research and Innovation, School of Applied and Life Sciences, Uttaranchal University, Premnagar, Dehradun, Uttarakhand, 248007, India
Ashutosh Kumar Rai
Department of Biochemistry, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, 31441, Kingdom of Saudi Arabia
Sheikh Shreaz
Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, PO Box 24885, 13109, Safat, Kuwait
Ajar Nath Yadav
Department of Genetics, Plant Breeding and Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour,173101, Himachal Pradesh, India; Corresponding author. Department of Genetics, Plant Breeding and Biotechnology, Dr. Khem Sigh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour, 173101, Himachal Pradesh, India.
Microbes are a worthwhile organism of the earth that could be formulated as consortium which can be utilized as biofertilizers. Consortium-based bioinoculants or biofertilizers are superior to single strain-based inoculants for sustainable agricultural productivity and increased micronutrient content in yield. The aim of present study was to evaluate the effect of different combinations of beneficial bacteria that are more effective than single-based bioinoculants. The current work focuses on the isolation of rhizospheric microorganisms from various cereals and pseudocereal crops and the development of a single inoculum as well as a bacterial consortium which were evaluated on wheat crop. A total 214 rhizospheric bacteria were sorted out and, screened for mineral solubilizing attributes i.e., phosphorus, potassium, zinc and selenium solubilization. Among all the bacterial isolates, four potential strains exhibiting P, K, Zn and Se-solubilizing attributes were identified with the help of 16S rRNA gene sequencing as Rahnella aquatilis EU-A3Rb1, Erwinia aphidicola EU-A2RNL1, Brevibacillus brevis EU-C3SK2, and Bacillus mycoides EU-WRSe4, respectively. The identified strains formulated as a consortium which were found to improve the plant growth and physiological parameters in comparison to single culture inoculants and control. To the best of our knowledge, the present investigation is the first report that has developed the consortium from bacterial strains Rahnella aquatilis EU-A3Rb1, Erwinia aphidicola EU-A2RNL1, Brevibacillus brevis EU-C3SK2, and Bacillus mycoides EU-WRSe4. A combination of bacterial strains could be used as liquid inoculants for cereal crops growing in mountainous regions.
