Mìkrobìologìâ ì Bìotehnologìâ (Dec 2019)
INFLUENCE OF NICOTINOYLHYDRAZONES AND Ge(IV) AND Sn(IV) COMPLEXES BASED ON THEM ON THE GROWTH OF PHYTOPATHOGENIC FUNGI
Abstract
Aim. To evaluate the influence of nicotinoylhydrazones with different molecule structure and complexes of Ge(IV) and Sn(IV) based on them on the growth of phytopathogenic fungi. Меthods. 2-hydroxy-1-naphthaldehyde nicotinoylhydrazone and 2-hydroxybenzaldehyde nicotinoylhydrazone and corresponding complexes of Ge(IV) and Sn(IV) were used in the study. Antifungal activity of studied compounds towards phytopathogenic fungi Alternaria alternata (Fr.) Keissl. F11, Botrytis cinerea Pers. F12, Pyrenophora teres Drechsler F13, Fusarium graminearum Schwabe F14, Ceratorhiza cerealis (E.P. Hoeven) R.T. Moore F15, Sclerotinia sclerotiorum (Lib.) de Bary F16 was evaluated by measurement of fungal colony diameter on the 3rd, 7th and 10th day of cultivation, and also by serial dilution method in Sabouraud broth (range of concentrations – from 25 to 100 µM). Results. Evaluation of fungal colony diameters demonstrated that studied compounds at the concentration 25 µM were able to cause significant decrease (by 17–54% compared to control) of growth of pathogen colonies. Also, mycelium development disorders were observed in Sabouraud broth at the presence of 15, 50 and 100 µM of hydrazones and metal-complexes. The most susceptible was C. cerealis F15, which growth was significantly (from 31.8 to 54.6%) inhibited by all the compounds at the concentration 25 µM, and also S. sclerotiorum (5 compounds) and A. alternata – 4 of 6 compounds. The complexes of hydrazones with tin and germanium showed more significant activity and wider antifungal spectrum compared to hydrazones they are based on. Conclusion. Studied hydrazones and based on them complexes of Ge(IV) and Sn(IV) inhibit the growth of phytopathogenic fungi which belong to both ascomycetes (Alternaria alternata, Sclerotinia sclerotiorum) and basidiomycetes (Ceratorhiza cerealis).
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