Antibacterial and Antioxidant Activity of Endophytic Fungi Extract Isolated from Leaves of Sungkai (Peronema canescens)

Abstract

Sungkai is a plant that is widely found in Indonesia. This plant is often used in traditional medicine so the biotechnology of endophyticfungi is highly needed due to natural resources from plants that have been reduced. This study reported on endophytic fungi foundin sungkai leaves and the compound produced. Morphological and molecular identification through phylogenetic tree analysis wascarried out to determine the endophytic fungal species found. Potato Dextrose Broth media was used for the cultivation process.Ethyl acetate was used as a solvent for the extraction and the evaporation process used a rotary evaporator. Antioxidant andantibacterial tests were carried out using the DPPH method and paper disc diffusion. Chromatographic techniques were used toisolate the compound and spectroscopic analysis was performed to identify its chemical structure. The results of the morphologicaland molecular analysis showedTrichoderma asperellumas an endophytic fungus identified. The pure compound obtained from thisendophytic fungus was 4-hydroxybenzoic acid. The antioxidant and antibacterial activity showed a strong category (IC50 = 43.88𝜇g/mL; MIC 64𝜇g/mL). This compound was very likely to be a raw material for new antibiotics and antioxidants through furtherresearch with various modifications.

Keywords

• bioactive compound
• endophytic fungi
• trichoderma asperellum
• sungkai