Adoptive Transfer of Basophils Enriched from Mouse Spleen
Adrian Piliponsky,
Stephen Galli
Affiliations
Adrian Piliponsky
Center for Immunity and Immunotherapies, Seattle Children’s Research Institute, Seattle, WA, USADepartments of Pediatrics, University of Washington School of Medicine, Seattle, WA, USA, Departments of Pathology, University of Washington School of Medicine, Seattle, WA, USA
Stephen Galli
Department of Pathology, Stanford University School of Medicine, Stanford, CA, USASean N. Parker Center for Allergy and Asthma Research, Stanford University School of Medicine, CA, USA, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA
CD49b is a member of the integrin family, expressed on basophils, natural killer (NK) cells and a subset CD4+ T cells in the spleen. This protocol describes the adoptive transfer of basophil-enriched CD49b+ cells obtained from mouse spleens by magnetic enrichment. This protocol can be used to assess the contribution of basophils or basophil-derived mediators to a certain immune response.
