LncRNA SNHG5 adversely governs follicular growth in PCOS via miR-92a-3p/CDKN1C axis
Zuwei Yang,
Jiexue Pan,
Chengliang Zhou,
Chuanjin Yu,
Zhiyang Zhou,
Guolian Ding,
Xinmei Liu,
Jianzhong Sheng,
Li Jin,
Hefeng Huang
Affiliations
Zuwei Yang
Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Research Units of Embryo Original Diseases, Chinese Academy of Medical Sciences (No. 2019RU056), Shanghai, China
Jiexue Pan
Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China; Research Units of Embryo Original Diseases, Chinese Academy of Medical Sciences (No. 2019RU056), Shanghai, China
Chengliang Zhou
The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Chuanjin Yu
Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China
Zhiyang Zhou
Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China
Guolian Ding
Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China
Xinmei Liu
Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China
Jianzhong Sheng
Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China
Li Jin
Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China; Research Units of Embryo Original Diseases, Chinese Academy of Medical Sciences (No. 2019RU056), Shanghai, China; Corresponding author
Hefeng Huang
Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Research Units of Embryo Original Diseases, Chinese Academy of Medical Sciences (No. 2019RU056), Shanghai, China; Shanghai Key Laboratory of Reproduction and Development, Shanghai, China; Key Laboratory of Reproductive Genetics (Ministry of Education), Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China; Corresponding author
Summary: Small nucleolar RNA host genes (SNHGs) have been implicated in various biological processes, yet their involvement in polycystic ovary syndrome (PCOS) remains elusive. Specifically, SNHG5, a long non-coding RNA implicated in several human cancers, shows elevated expression in granulosa cells (GCs) of PCOS women and induces PCOS-like features when overexpressed in mice. In vitro, SNHG5 inhibits GC proliferation and induces apoptosis and cell-cycle arrest at G0/G1 phase, with RNA-seq indicating its impact on DNA replication and repair pathways. Mechanistically, SNHG5 acts as a competing endogenous RNA by binding to miR-92a-3p, leading to increased expression of target gene CDKN1C, which further suppresses GC proliferation and promotes apoptosis. These findings elucidate the crucial role of SNHG5 in the pathogenesis of PCOS and suggest a potential therapeutic target for this condition. Additional investigations such as large-scale clinical studies and functional assays are warranted to validate and expand upon these findings.
