Effect of Pseudomonas Fluorescens on Isofetamid Dissipation and Soil Microbial Activity

Abstract

The aim of this study was to assess the effect of Pseudomonas fluorescens (P) application on isofetamid (IS) dissipation; the number of specific genes of archaea, bacteria and ammonia-oxidizing bacteria (AOB); and the activity of β-Glucosidase, phosphomonoesterase, N-acetyl-glucosaminidase and arylsulfatase. It was observed that the IS concentration was lower in the P+IS than in IS throughout the entire study period, which indicates the potential of P. fluorescens to decompose isofetamid faster. IS+P application significantly influenced N-acetyl-glucosaminidase, arylsulfatase and phosphomonoesterase activity in soil compared to the control by approximately 29%, 72% and 6.5%, respectively. Moreover, it was observed that on day 21 in IS+P, the number of bacterial genes was significantly higher than in the control and IS and than on day 1, by 10% and 20%, respectively. On day 21, the number of archaea was significantly higher in all variants and ranged from 3.61 (control) to 6.88 log10 gene copies/g dm (IS+P). Correlation analysis showed a positive correlation between IS and TOC, while there was a negative correlation between IS and β-Glu and the number of archaea and AOB genes. The tested strain has the potential to be a biofertilizer and an agent in the bioremediation of contaminated soils.

Keywords

• isofetamid
• soil enzymes
• <i>Pseudomonas fluorescens</i>
• functional genes