Improved spot morphology for printed glycan arrays
Navakouski Maksim,
Shilova Nadezhda,
Khasbiullina Nailya,
Feofanov Alexey,
Pudova Elena,
Chen Kowa,
Blixt Ola,
Bovin Nicolai
Affiliations
Navakouski Maksim
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997 Russia
Shilova Nadezhda
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997 Russia
Khasbiullina Nailya
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997 Russia
Feofanov Alexey
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997 Russia
Pudova Elena
3Central Research Institute of Epidemiology, Federal Service for Supervision of Consumer rights Protection and Human Well-Being, Novogireevskaia 3a, Moscow, 111123 Russia
Chen Kowa
4Department of chemistry, University of Copenhagen, t422, Thorvaldsensvej 40, Frederiksberg, 1870 Denmark
Blixt Ola
4Department of chemistry, University of Copenhagen, t422, Thorvaldsensvej 40, Frederiksberg, 1870 Denmark
Bovin Nicolai
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997 Russia
Despite considerable success studying glycan-binding proteins using printed glycan arrays (PGAs), unambiguous quantitation of spot intensities by fluorescent readers remains a challenge. The main obstacles are the varying spot shape and size and in-spot fluorescence distribution caused by uneven drying of the printed drops. Two methods have been suggested for solving this problem: using polymeric glycoconjugates, which makes it possible to equalize the physicochemical properties (hydrophobicity, charge, and size) of different glycans, and applying a glycan solution on a slide coated with a thin oil mask, which hinders evaporation of the drop. Both approaches yield spots with similar sizes and an even distribution of the signal across the spot and are likely to be useful for improving the prints of other classes of molecules.
