A Multiplexed Microfluidic Platform for Bone Marker Measurement: A Proof-of-Concept
Patricia Khashayar,
Ghassem Amoabediny,
Bagher Larijani,
Morteza Hosseini,
Rik Verplancke,
David Schaubroeck,
Steven Van Put,
Farideh Razi,
Michel De Keersmaecker,
Annemie Adriaens,
Stefan Goemaere,
Tom Fiers,
Jan Vanfleteren
Affiliations
Patricia Khashayar
Nanobiotechnology Department, Faculty of New Sciences & Technologies, University of Tehran, Tehran 1439957131, Iran
Ghassem Amoabediny
Department of Biotechnology, Faculty of Chemical Engineering, School of Engineering, University of Tehran, Tehran 1417614418, Iran
Bagher Larijani
Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran 1411413137, Iran
Morteza Hosseini
Nanobiotechnology Department, Faculty of New Sciences & Technologies, University of Tehran, Tehran 1439957131, Iran
Rik Verplancke
Center for Microsystems Technology, Imec and Ghent University, Gent-Zwijnaarde 9052, Belgium
David Schaubroeck
Center for Microsystems Technology, Imec and Ghent University, Gent-Zwijnaarde 9052, Belgium
Steven Van Put
Center for Microsystems Technology, Imec and Ghent University, Gent-Zwijnaarde 9052, Belgium
Farideh Razi
Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran 1411413137, Iran
Michel De Keersmaecker
Department of Analytical Chemistry, Ghent University, Krijgslaan 281 S12, Ghent 9052, Belgium
Annemie Adriaens
Department of Analytical Chemistry, Ghent University, Krijgslaan 281 S12, Ghent 9052, Belgium
Stefan Goemaere
Unit for Osteoporosis and Metabolic Bone Diseases, Ghent University Hospital, Ghent 9052, Belgium
Tom Fiers
Unit for Osteoporosis and Metabolic Bone Diseases, Ghent University Hospital, Ghent 9052, Belgium
Jan Vanfleteren
Center for Microsystems Technology, Imec and Ghent University, Gent-Zwijnaarde 9052, Belgium
In this work, we report a microfluidic platform that can be easily translated into a biomarker diagnostic. This platform integrates microfluidic technology with electrochemical sensing and embodies a reaction/detection chamber to measure serum levels of different biomarkers. Microfabricated Au electrodes encased in a microfluidic chamber are functionalized to immobilize the antibodies, which can selectively capture the corresponding antigen. An oxidative peak is obtained using the chronoamperometry technique at room temperature. The magnitude of the response current varies linearly with the logarithmic concentration of the relative biomarker and, thus, is used to quantify the concentration of the relative biomarker in serum samples. We demonstrated the implementation, feasibility and specificity of this platform (Osteokit) in assaying serum levels of bone turnover markers (BTMs) using osteocalcin (limits of detection (LOD) = 1.94 ng/mL) and collagen type 1 cross-linked C-telopeptide (CTX) (LOD = 1.39 pg/mL). To our knowledge, this is the first such device fabricated to measure BTMs. Our results also showed that the sensitivity of Osteokit is comparable with the current states of art, electrochemiluminescence (ECLIA).
