Influence of dissolved oxygen in the environment on toxicity indices obtained by various bio-testing methods

Abstract

RELEVANCE of the study comes from the understanding of the necessity of continuous development of toxicological analysis. Since The simplest organisms are used in toxicological analysis as bioindicators, the reactions of which to the presence of certain pollutants will always differ, the only way to assess the usefulness of a bioindicators as a tool is to study its behavior under specific tasks. Biondicators differ in their metabolic properties, in particular, in the ways of obtaining energy from the presence of molecular oxygen in the environment. However, it is assumed that the presence of different concentrations of oxygen in the environment affects the behavior of the simplest organisms, which, in its turn, can affect the results of toxicological analysis. Previously, this effect has not been studied. THE PURPOSE. The research of the effect of dissolved oxygen on toxicity indices obtained using of bacteria Escherichia coli (Migula) Castellani and Chalmers and ciliates Paramecium caudatum Ehrenberg, METHODS. Obtaining indices of toxicity is given with different concentrations of dissolved oxygen in the environment, which are distilled water and Bonaqua brand water, by the bioluminescent reaction of bacteria and chemotactic reaction of ciliates. RESULTS. With an increase in the concentration of dissolved oxygen in the environment, the bioluminescence of bacteria increases, therefore, the toxicity index decreases. No effect on ciliates is observed. CONCLUSION. It can be concluded that dissolved oxygen does not affect the biotest analysis using ciliates. When using bacteria as a test organism, an error occurs in the toxicity index.

Keywords

• dissolved oxygen
• toxicity
• bioassay
• escherichia coli
• paramecium caudatum