Nature Communications (Jan 2018)

Intelligent image-based in situ single-cell isolation

  • Csilla Brasko,
  • Kevin Smith,
  • Csaba Molnar,
  • Nora Farago,
  • Lili Hegedus,
  • Arpad Balind,
  • Tamas Balassa,
  • Abel Szkalisity,
  • Farkas Sukosd,
  • Katalin Kocsis,
  • Balazs Balint,
  • Lassi Paavolainen,
  • Marton Z. Enyedi,
  • Istvan Nagy,
  • Laszlo G. Puskas,
  • Lajos Haracska,
  • Gabor Tamas,
  • Peter Horvath

DOI
https://doi.org/10.1038/s41467-017-02628-4
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 7

Abstract

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The isolation of single cells while retaining context is important for quantifying cellular heterogeneity but technically challenging. Here, the authors develop a high-throughput, scalable workflow for microscopy-based single cell isolation using machine-learning, high-throughput microscopy and laser capture microdissection.