Review of Chromatography Methods for Purfication of Paraoxonase Enzyme

Abstract

Human serum paraoxonase1 hPON1 is a calcium dependent enzyme, which has an important physiological role in organism with detoxification, antioxidant and also antiatherogenic properties. PON1 is mostly synthesized from the liver than secreted in serum with located on HDL. In order to better understand the molecular mechanism of paraoxonase 1, researchers have been increasingly interested in the development of various methods for obtaining the enzyme in pure form over the last decades. In this paper, several different purification and characterization methods of multi functional enzyme paraoxonase 1 EC 3.3.8.1 was reviewed.

Keywords

• paraoxonase1
• chromatography methods
• enzyme purification and characterization