Multiplexed single-molecule force spectroscopy using a centrifuge

Darren Yang; Andrew Ward; Ken Halvorsen; Wesley P. Wong

doi:10.1038/ncomms11026

Nature Communications (Mar 2016)

Multiplexed single-molecule force spectroscopy using a centrifuge

Darren Yang,
Andrew Ward,
Ken Halvorsen,
Wesley P. Wong

Affiliations

Darren Yang: School of engineering and applied sciences, Harvard University
Andrew Ward: Program in Cellular and Molecular Medicine, Boston Children’s Hospital
Ken Halvorsen: Program in Cellular and Molecular Medicine, Boston Children’s Hospital
Wesley P. Wong: Program in Cellular and Molecular Medicine, Boston Children’s Hospital

DOI: https://doi.org/10.1038/ncomms11026
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 7

Abstract

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Single molecule force spectroscopy (SMFS) has limitations in throughput and the ability to repeatedly interrogate single bonds. Here the authors repurpose a benchtop centrifuge and use DNA nanoswitches to enable high throughput SMFS capable of repeatedly measuring forces of single molecular pairs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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