Synthesis and characterization of gold nano particles using barleria cristata for antimicrobial and anticancer applications

Abstract

A nano revolution is necessary for the integration of nanoscience and medicine. Drug delivery, electronics, biological sciences, optics, and catalysis are among the fields in which metal nanoparticles find application. The objective of this work is to extract leaves from Barleria cristata in order to produce ecologically friendly gold nanoparticles (Au NP) for pharmacological studies. Several human pathogens were used to assess the biological mechanism and antibacterial properties of gold nanoparticles (AuNPs) in relation to their potential as a cancer treatment. An aqueous extract with a natural pH of 7.4 was obtained by reacting with 1 mM chloroauric acid (HAuCl4.3H2O), and it was then kept at room temperature. The colour changed from pale yellow to pink right away, indicating the reduction of Au3+ ions to Au 0. Using a UV-Visible spectrophotometer, the synthesised AuNPs were seen. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), field emission transmission electron microscopy (FE-TEM), and particle size distribution and zeta potential analysis are a few examples of analytical techniques. The disc diffusion method was used to confirm the extract's antibacterial properties in vitro on a few human illnesses. The MTT test was used to confirm the extract's anticancer properties on Hela carcinoma cell lines, revealing IC50 values of 50 μg/mL.