Polyvinyl alcohol/Polyvinylpyrrolidone/Chitosan Nanofiber Scaffold with Hydroxyapatite from Sand Lobster Shells (Panulirus homarus) for Bone Tissue Engineering

Abstract

In this work, nanofiber scaffold membrane polyvinyl alcohol (PVA)/polyvinylpyrrolidone (PVP)/Chitosan (CS)/hydroxyapatite (HAp) from sand lobster (SL; Panulirus homarus) shells have successfully synthesized to mimic the extracellular matrix (ECM) nanoscale in the native bone. HAp was synthesized by co-precipitation method with Ca/P was 1.67, then nanofiber membrane PVA/PVP/CS/HAp was synthesized by electrospinning method. Nanofiber solution was prepared from PVA 10% (w/v) polymer solution that dissolved in the distilled water, then the PVP/CS 15% (w/v) polymer solution was dissolved in acetic acid 1% (v/v) separately. The PVA polymer solution and PVP/CS solution were mixed with a ratio of 8.5: 1.5 (v/v). HAp dispersed into mixture solution with variation concentration 0, 1, 3, and 5 wt%. The composite solution was put into a 10 ml syringe with a hole diameter = 0.5 mm. Electrospinning was carried out at a 10 kV voltage, the flow rate at 0.1 ml/h, and the distance between the collector to the tip was 12 cm. Nanofiber scaffold membrane was characterized using SEM, FTIR, and XRD. The addition of HAp into the fiber showed incorporation into nanofiber with small agglomeration in the concentration of HAp at 1, 3, and 5 wt%. Based on the physicochemical analysis, the nanofiber scaffold PVA/PVP/CS/HAp 5 wt% with a fiber diameter of 0.328 ± 0.049 μm has the most potential to be used for bone tissue engineering.