Isolation and Characterization of Antibiotic-producing Endophytic Bacteria from Citrus aurantifolia Swingle

Abstract

Research on the isolation and characterization of antibiotics-producing endophytic bacteria from Citrus aurantifolia leaves has been done. The isolation process was carried out by spreading spread plate isolation technique, screening of antibiotic-producing bacteria was done by disc dilution method and characterization of antibiotic-producing bacteria was done by microscopic, macroscopic and 16S rRNA analysis methods. The result of this research is four endophytic bacteria isolates that potentially produces antibiotic compounds. First, isolates with the CA01 code of fermentation fluids have inhibitory activity against Streptococcus mutans bacteria. Second, isolate with the code CA 02 able to inhibit growth of Vibrio chloreae bacteria. Third, isolates with the CA 03 code were able to inhibit bacterial growth of Salmonella thypii and E. faecalis. Fourth, isolate with CA 04 code has inhibitory activity on Salmonella thypii and Salmonella thyposa bacteria. The microscopic, macroscopic and 16S rRNA characterization for antibiotic-producing endophytic bacteria results were Bacillus cereus RNS_01 (code CA01), Pantoea agglomerans ZFJ-15 (Code CA 02), Bacillus subtilis 55C1-1 (Code CA 03) and Bacillus pumilus SH-B11 (Code CA 04).

Keywords

• endophytic bacteria
• citrus aurantifolia
• antibiotic
• 16s rrna