Discovery of a lectin domain that regulates enzyme activity in mouse N-acetylglucosaminyltransferase-IVa (MGAT4A)

Masamichi Nagae; Tetsuya Hirata; Hiroaki Tateno; Sushil K. Mishra; Noriyoshi Manabe; Naoko Osada; Yuko Tokoro; Yoshiki Yamaguchi; Robert J. Doerksen; Toshiyuki Shimizu; Yasuhiko Kizuka

doi:10.1038/s42003-022-03661-w

Communications Biology (Jul 2022)

Discovery of a lectin domain that regulates enzyme activity in mouse N-acetylglucosaminyltransferase-IVa (MGAT4A)

Masamichi Nagae,
Tetsuya Hirata,
Hiroaki Tateno,
Sushil K. Mishra,
Noriyoshi Manabe,
Naoko Osada,
Yuko Tokoro,
Yoshiki Yamaguchi,
Robert J. Doerksen,
Toshiyuki Shimizu,
Yasuhiko Kizuka

Affiliations

Masamichi Nagae: Department of Molecular Immunology, Research Institute for Microbial Diseases, Osaka University
Tetsuya Hirata: Institute for Glyco-core Research (iGCORE), Gifu University
Hiroaki Tateno: Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
Sushil K. Mishra: Glycoscience Center of Research Excellence, Department of BioMolecular Sciences, University of Mississippi, University
Noriyoshi Manabe: Division of Structural Glycobiology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University
Naoko Osada: Institute for Glyco-core Research (iGCORE), Gifu University
Yuko Tokoro: Institute for Glyco-core Research (iGCORE), Gifu University
Yoshiki Yamaguchi: Division of Structural Glycobiology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University
Robert J. Doerksen: Glycoscience Center of Research Excellence, Department of BioMolecular Sciences, University of Mississippi, University
Toshiyuki Shimizu: Faculty of Pharmaceutical Sciences, The University of Tokyo
Yasuhiko Kizuka: Institute for Glyco-core Research (iGCORE), Gifu University

DOI: https://doi.org/10.1038/s42003-022-03661-w
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 11

Abstract

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X-ray crystallography together with NMR and computer modelling shed light on the structure and catalytic mechanism of GnTIVa, a key enzyme involved in GlcNAc branch synthesis, that bears an unusual C-terminal lectin domain that regulates its catalytic activity.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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