STAR Protocols (Jun 2021)

Protocol for determining protein cysteine thiol redox status using western blot analysis

  • Bikram Datt Pant,
  • Sunhee Oh,
  • Kirankumar S. Mysore

Journal volume & issue
Vol. 2, no. 2
p. 100566

Abstract

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Summary: This protocol describes the analysis of protein cysteine redox status. Redox status is crucial in regulating protein activity, stability, and redox signaling cascades. It is determined by conjugation with 1.24 kDa MM(PEG)24 molecule to each reduced cysteine followed by western blot analysis. This protocol is easy to follow, and most of the reagents and instruments required are of common use in any lab. This protocol can be successfully applied to other biological sources.For complete details on the use and execution of this protocol, please refer to Pant et al. (2020).

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