Antimicrobial Activity of Peptides Produced by <i>Lactococcus lactis</i> subsp. <i>lactis</i> on Swine Pathogens
Fernando M. M. Sanca,
Iago R. Blanco,
Meriellen Dias,
Andrea M. Moreno,
Simone M. M. K. Martins,
Marco A. Stephano,
Maria A. Mendes,
Carlos M. N. Mendonça,
Wellison A. Pereira,
Pamela O. S. Azevedo,
Martin Gierus,
Ricardo P. S. Oliveira
Affiliations
Fernando M. M. Sanca
Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil
Iago R. Blanco
Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil
Meriellen Dias
Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil
Andrea M. Moreno
Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo 05508-000, Brazil
Simone M. M. K. Martins
Department of Animal Sciences, Faculty of Animal Sciences and Food Engineering, University of São Paulo, São Paulo 05508-000, Brazil
Marco A. Stephano
Immunobiological and Biopharmaceutical Laboratory, School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, Brazil
Maria A. Mendes
Dempster Mass Spectrometry Laboratory, Chemical Engineering Department, Polytechnic School, University of São Paulo, São Paulo 05508-000, Brazil
Carlos M. N. Mendonça
Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil
Wellison A. Pereira
Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil
Pamela O. S. Azevedo
Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil
Martin Gierus
Institute of Animal Nutrition, Livestock Products, and Nutrition Physiology (TTE), Department of Agrobiotechnology, IFA-Tulln, University of Natural Resources and Life Sciences, 1190 Vienna, Austria
Ricardo P. S. Oliveira
Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil
Swine production is of great importance worldwide and has huge economic and commercial impact. Due to problems with bacterial infection, the use of antimicrobials has increased in the last decades, particularly in Latin America and Asia. This has led to concerns about antimicrobial resistance, which poses risks to human health and the environment. The use of probiotic organisms has been proposed as an alternative to this use, as these beneficial bacteria can produce antimicrobial peptides, such as bacteriocins, which allow the induction of inhibitory effects against pathogenic microorganisms. Among probiotics, some bacteria stand out with the inhibition of animal pathogens. The bacteriocin-like inhibitory substances (BLISs) of Lactococcus lactis subsp. lactis strain L2, present in its cell-free supernatant, were tested against pathogenic strains isolated from pig samples, such as Escherichia coli, Salmonella enterica, Streptococcus suis, Streptococcus dysgalactiae, Staphylococcus hyicus, and Enterococcus faecalis. Compounds secreted by L. lactis L2 have been shown to inhibit the growth of some pathogenic species, particularly Gram-positive bacteria, with S. suis being the most prominent. Antimicrobial peptides with a molecular size of 500–1160 Daltons were isolated from BLISs. The results highlight the potential of L. lactis BLISs and its peptides as natural antimicrobials for use in the food industry and to reduce the use of growth promoters in animal production.