Preparation of long single-strand DNA concatemers for high-level fluorescence in situ hybridization

Dongjian Cao; Sa Wu; Caili Xi; Dong Li; Kaiheng Zhu; Zhihong Zhang; Hui Gong; Qingming Luo; Jie Yang

doi:10.1038/s42003-021-02762-2

Communications Biology (Oct 2021)

Preparation of long single-strand DNA concatemers for high-level fluorescence in situ hybridization

Dongjian Cao,
Sa Wu,
Caili Xi,
Dong Li,
Kaiheng Zhu,
Zhihong Zhang,
Hui Gong,
Qingming Luo,
Jie Yang

Affiliations

Dongjian Cao: Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology
Sa Wu: Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology
Caili Xi: Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology
Dong Li: Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology
Kaiheng Zhu: Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology
Zhihong Zhang: Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology
Hui Gong: Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology
Qingming Luo: Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology
Jie Yang: Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology

DOI: https://doi.org/10.1038/s42003-021-02762-2
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 9

Abstract

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Cao et al. present a method to prepare long single-strand DNA concatemers (lssDNAc) through a controllable rolling-circle amplification (CRCA), used to develop AmpFISH workflows. Their method is suitable for labelling to both individual mRNA molecules and chromosomes and can also be multiplexed with immunofluorescence, allowing for sensitive RNA/DNA detection.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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