Nature Communications (Aug 2020)
Multiplexed single-cell transcriptional response profiling to define cancer vulnerabilities and therapeutic mechanism of action
- James M. McFarland,
- Brenton R. Paolella,
- Allison Warren,
- Kathryn Geiger-Schuller,
- Tsukasa Shibue,
- Michael Rothberg,
- Olena Kuksenko,
- William N. Colgan,
- Andrew Jones,
- Emily Chambers,
- Danielle Dionne,
- Samantha Bender,
- Brian M. Wolpin,
- Mahmoud Ghandi,
- Itay Tirosh,
- Orit Rozenblatt-Rosen,
- Jennifer A. Roth,
- Todd R. Golub,
- Aviv Regev,
- Andrew J. Aguirre,
- Francisca Vazquez,
- Aviad Tsherniak
Affiliations
- James M. McFarland
- Broad Institute of MIT and Harvard
- Brenton R. Paolella
- Broad Institute of MIT and Harvard
- Allison Warren
- Broad Institute of MIT and Harvard
- Kathryn Geiger-Schuller
- Broad Institute of MIT and Harvard
- Tsukasa Shibue
- Broad Institute of MIT and Harvard
- Michael Rothberg
- Broad Institute of MIT and Harvard
- Olena Kuksenko
- Broad Institute of MIT and Harvard
- William N. Colgan
- Broad Institute of MIT and Harvard
- Andrew Jones
- Broad Institute of MIT and Harvard
- Emily Chambers
- Broad Institute of MIT and Harvard
- Danielle Dionne
- Broad Institute of MIT and Harvard
- Samantha Bender
- Broad Institute of MIT and Harvard
- Brian M. Wolpin
- Harvard Medical School
- Mahmoud Ghandi
- Broad Institute of MIT and Harvard
- Itay Tirosh
- Klarman Cell Observatory, Broad Institute of MIT and Harvard
- Orit Rozenblatt-Rosen
- Broad Institute of MIT and Harvard
- Jennifer A. Roth
- Broad Institute of MIT and Harvard
- Todd R. Golub
- Broad Institute of MIT and Harvard
- Aviv Regev
- Broad Institute of MIT and Harvard
- Andrew J. Aguirre
- Broad Institute of MIT and Harvard
- Francisca Vazquez
- Broad Institute of MIT and Harvard
- Aviad Tsherniak
- Broad Institute of MIT and Harvard
- DOI
- https://doi.org/10.1038/s41467-020-17440-w
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 15
Abstract
Large-scale screens of chemical and genetic vulnerabilities in cancer are typically limited to simple readouts of cell viability. Here, the authors develop a method for profiling post-perturbation transcriptional responses across large pools of cancer cell lines, enabling deep characterization of shared and context-specific responses.
