Nature Communications (Apr 2018)

Methanol-essential growth of Escherichia coli

  • Fabian Meyer,
  • Philipp Keller,
  • Johannes Hartl,
  • Olivier G. Gröninger,
  • Patrick Kiefer,
  • Julia A. Vorholt

DOI
https://doi.org/10.1038/s41467-018-03937-y
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 10

Abstract

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Engineering synthetic methylotrophy remains challenging. Here, the authors engineer a methanol-essential E. coli by an in silico-guided multiple knockout approach and show a laboratory evolved strain can incorporate up to 24% methanol into core metabolites during growth.