Agro-industrial Residues: Low-cost Biomass for the Production of Bioactive Extracellular Polysaccharides by Lactobacillus plantarum in Solid State Fermentation
Hissah Abdulrahman Alodaini,
Rajamony Rajam Sheeja,
Joseph Joselin,
Ashraf Atef Hatamleh,
Munirah Abdullah Al-Dosary,
Sundaramani Alci Rani,
Ponnuswamy Vijayaraghavan,
Selvaraj Arokiyaraj
Affiliations
Hissah Abdulrahman Alodaini
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Rajamony Rajam Sheeja
Department of Botany, Women’s Christian College (Affiliated to Manonmanium Sundaranar University, Tirunelveli - 627 012
Joseph Joselin
Department of Botany, Women’s Christian College (Affiliated to Manonmanium Sundaranar University, Tirunelveli - 627 012), Nagercoil, Kanniyakumari District, Tamil Nadu, India – 629 001
Ashraf Atef Hatamleh
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Munirah Abdullah Al-Dosary
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Sundaramani Alci Rani
Department of Botany, Nesamony Memorial Christian College (Affiliated to Manonmanium Sundaranar University, Tirunelveli - 627 012), Marthandam, Kanniyakumari District, Tamil Nadu, India – 629 165
Agro-residues, such as apple peel, banana peel, orange peel, tapioca peel, and sweet potato peel powders, were screened for the biosynthesis of bioactive extracellular polysaccharide (EPS) in solid-state fermentation. Lactobacillus plantarum utilized sweet potato peel and improved EPS production compared with other wastes, so it was used for optimization studies. Compared with the unoptimized medium, the response surface methodology improved EPS production twofold during solid-state fermentation. In the central composite design, the experimental model was significant (p<0.01), and the selected variables (moisture, sucrose and yeast extract) significantly influenced EPS production. The maximum yield was 59.1 mg/g with 64.5% moisture content, 5.74% sucrose, and 1.39% yeast extract. In addition, the extracted EPS exhibited strong antioxidant activity, and it scavenged ABTS, DPPH, and hydroxyl radicals in a concentration-dependent manner. The antibacterial activity of EPS was greatest against Staphylococcus aureus, and the corresponding zone of inhibition was18±1 mm, followed by Pseudomonas aeruginosa(14±1 mm).The results showed that sweet potato peels can be used as a cheap substrate for EPS production in solid-state fermentation.
