Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation

Yu Yan; Jing Wu; Guipeng Hu; Cong Gao; Liang Guo; Xiulai Chen; Liming Liu; Wei Song

Synthetic and Systems Biotechnology (Sep 2022)

Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation

Yu Yan,
Jing Wu,
Guipeng Hu,
Cong Gao,
Liang Guo,
Xiulai Chen,
Liming Liu,
Wei Song

Affiliations

Yu Yan: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China
Jing Wu: School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China
Guipeng Hu: School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China
Cong Gao: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China
Liang Guo: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China
Xiulai Chen: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China
Liming Liu: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China
Wei Song: School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China; Corresponding author.

Journal volume & issue: Vol. 7, no. 3
pp. 887 – 899

Abstract

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Cytochrome P450 enzymes (CYPs) catalyze a series of C–H and C=C oxygenation reactions, including hydroxylation, epoxidation, and ketonization. They are attractive biocatalysts because of their ability to selectively introduce oxygen into inert molecules under mild conditions. This review provides a comprehensive overview of the C–H and C=C oxygenation reactions catalyzed by CYPs and the various strategies for achieving higher selectivity and enzymatic activity. Furthermore, we discuss the application of C–H and C=C oxygenation catalyzed by CYPs to obtain the desired chemicals or pharmaceutical intermediates in practical production. The rapid development of protein engineering for CYPs provides excellent biocatalysts for selective C–H and C=C oxygenation reactions, thereby promoting the development of environmentally friendly and sustainable production processes.

Published in Synthetic and Systems Biotechnology

ISSN: 2405-805X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/synthetic-and-systems-biotechnology/

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Abstract

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