Detection of QTLs for cold tolerance at the booting stage in near-isogenic lines derived from rice landrace Lijiangxintuanheigu

Abstract

Chilling damage significantly reduces grain yield in rice, while exploring major quantitative trait loci (QTLs) has the potential to improve rice production. Mapping of QTLs for 5 cold tolerance-related traits at the booting stage was conducted with SSR markers and inclusive composite interval mapping (ICIM) approach, based on 105 near-isogenic lines derived from a backcross between Lijiangxintuanheigu (LTH, cold-tolerant landrace) and Towada (cold-sensitive cultivar). Phenotype values were investigated under five cold-stress environments and analysed by the best linear unbiased prediction (BLUP). Twenty-one QTLs were identified on chromosomes 1, 2, 3, 4, 6, 7, 10 and 11, and the amount of variation (R2) explained by each QTL ranged from 7.71 to 29.66%, with five co-located QTL regions. Eight novel major loci (qSF-2, qSF-6a, qSF-7, qGW-6, qDGWP-4, qDSWPP-4, qDWPP-1 and qDWPP-4b) were detected in several environments and BLUP, and their alleles were contributed by LTH with R2 variance from 12.24 to 29.66%. These favourable QTLs would facilitate elucidation of the genetic mechanism of cold tolerance and provide strategies for breeding high-productive rice.

Keywords

• oryza sativa
• quantitative trait loci
• near-isogenic lines
• cold tolerance at the booting stage