A pathogenesis-based transcript signature in donor-specific antibody-positive kidney transplant patients with normal biopsies
P. Ó Broin,
N. Hayde,
Y. Bao,
B. Ye,
R.B. Calder,
G. de Boccardo,
M. Lubetzky,
M. Ajaimy,
J. Pullman,
A. Colovai,
E. Akalin,
A. Golden
Affiliations
P. Ó Broin
Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA
N. Hayde
Division of Pediatric Nephrology, University of Texas Health Science Center, Houston, TX, USA
Y. Bao
Montefiore–Einstein Center for Transplantation, Montefiore Medical Center, The University Hospital for Albert Einstein College of Medicine, Bronx, NY, USA
B. Ye
Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA
R.B. Calder
Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA
G. de Boccardo
Montefiore–Einstein Center for Transplantation, Montefiore Medical Center, The University Hospital for Albert Einstein College of Medicine, Bronx, NY, USA
M. Lubetzky
Montefiore–Einstein Center for Transplantation, Montefiore Medical Center, The University Hospital for Albert Einstein College of Medicine, Bronx, NY, USA
M. Ajaimy
Montefiore–Einstein Center for Transplantation, Montefiore Medical Center, The University Hospital for Albert Einstein College of Medicine, Bronx, NY, USA
J. Pullman
Department of Pathology, Albert Einstein College of Medicine, Bronx, NY, USA
A. Colovai
Montefiore–Einstein Center for Transplantation, Montefiore Medical Center, The University Hospital for Albert Einstein College of Medicine, Bronx, NY, USA
E. Akalin
Montefiore–Einstein Center for Transplantation, Montefiore Medical Center, The University Hospital for Albert Einstein College of Medicine, Bronx, NY, USA
A. Golden
Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA
Affymetrix Human Gene 1.0-ST arrays were used to assess the gene expression profiles of kidney transplant patients who presented with donor-specific antibodies (DSAs) but showed normal biopsy histopathology and did not develop antibody-mediated rejection (AMR). Biopsy and whole-blood profiles for these DSA-positive, AMR-negative (DSA+/AMR−) patients were compared to both DSA-positive, AMR-positive (DSA+/AMR+) patients as well as DSA-negative (DSA−) controls. While individual gene expression changes across sample groups were relatively subtle, gene-set enrichment analysis using previously identified pathogenesis-based transcripts (PBTs) identified a clear molecular signature involving increased rejection-associated transcripts in AMR− patients. Results from this study have been published in Kidney International (Hayde et al., 2014 [1]) and the associated data have been deposited in the GEO archive and are accessible via the following link: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE50084
