Antibacterial, antioxidant and neuroprotective activities of crude extract from the endophytic fungus Fusarium sp. isolate OQ-Fus-2-F from Euphorbia sp. plant

Abstract

Context: Human life is contested with the emergence of new infectious diseases and antibiotic-resistant pathogens, cellular deterioration due to oxidative stress, and age-related neurodegenerative disorders. Aims: To evaluate the antibacterial, antioxidant, and acetylcholinesterase inhibitory activities of crude extract from the endophytic fungus Fusarium sp. isolate OQ-Fus-2-F as a basis for medical application. Methods: Fusarium sp. isolate OQ-Fus-2-F was isolated from the stem of Euphorbia sp. plant. It was identified morphologically and based on 18S rRNA gene partial sequence. The isolated was cultivated in a malt extract glucose medium. Cell filtrate was extracted with ethyl acetate, and the crude extract was dried in vacuum. The crude extract was assessed for its antimicrobial activity through agar diffusion and micro-broth dilution assays; antioxidant activity was measured using DPPH and ABTS methods, and the anticholinergic activity was evaluated in terms of inhibition in acetylcholinesterase (AChE) activity. Results: Crude extract of Fusarium sp. isolate OQ-Fus-2-F was more potent in inhibiting the growth of B. subtilis with a MIC value of 125 µg/mL. Its antioxidant activity was highly detected in ABTS than DPPH assay with IC50 values 37.5 ± 3.5 µg/mL and 191.3 ± 17.6 µg/mL, respectively. Moreover, the extract inhibited the AChE activity with IC50 177.0 ± 13.7 µg/mL. Conclusions: Crude extract of Fusarium sp. isolate OQ-Fus-2-F has potential biomedical applications as it contains secondary metabolites with antimicrobial, antioxidant, and neuroprotective activates.

Keywords

• acetylcholinesterase inhibition
• alzheimer’s disease
• antibacterial activity
• antioxidant activity
• fusarium sp.