In-vivo effects of Green laser radiation on hematological parameters of albino rats using direct exposure methods.

Abstract

Laser beam has a thermal effect on the exposed body through oscillation of molecules. Rate of the vibrations depend on the time of exposure, intensity of the laser beam, the quantity of the absorbed dose, and the laser wavelength. Impacts of green laser beam on the hematological parameters of Albino Rats investigated in an in-vivo irradiation process. Green laser (200mW, 532nm) was used as a source of non-ionizing radiation to irradiate albino rats, the exposure carried out to heart position (in-vivo) of the rats directly. Fifteen female rats participated. Main blood components: white blood cells (WBC), red blood cells (RBC), and platelet (PLT) counts were evaluated using a direct exposure method for low and high radiation doses. High doses of laser beam affected the WBCs and PLTs significantly (P-value 0.05). Laser beam radiation affected on the main blood components at a limited time of exposure. Optimum radiation doses that had high effectiveness on blood component density depended on the type of blood components. As well as, high impacts (high significant) were on the density of PLT not RBCs.

Keywords

• rat
• hematological parameters
• green laser
• nonionizing radiation