Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet
Leroy C. Joseph,
Jianting Shi,
Quynh N. Nguyen,
Victoria Pensiero,
Chris Goulbourne,
Robert C. Bauer,
Hanrui Zhang,
John P. Morrow
Affiliations
Leroy C. Joseph
Department of Medicine, College of Physicians and Surgeons of Columbia University, 650 W 168th Street, New York, NY 10032, USA
Jianting Shi
Department of Medicine, College of Physicians and Surgeons of Columbia University, 650 W 168th Street, New York, NY 10032, USA; Cardiometabolic Genomics Program, Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
Quynh N. Nguyen
Department of Medicine, College of Physicians and Surgeons of Columbia University, 650 W 168th Street, New York, NY 10032, USA
Victoria Pensiero
Department of Medicine, College of Physicians and Surgeons of Columbia University, 650 W 168th Street, New York, NY 10032, USA
Chris Goulbourne
Center for Dementia Research, Nathan S. Kline Institute, Orangeburg, NY, USA
Robert C. Bauer
Department of Medicine, College of Physicians and Surgeons of Columbia University, 650 W 168th Street, New York, NY 10032, USA
Hanrui Zhang
Department of Medicine, College of Physicians and Surgeons of Columbia University, 650 W 168th Street, New York, NY 10032, USA; Cardiometabolic Genomics Program, Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
John P. Morrow
Department of Medicine, College of Physicians and Surgeons of Columbia University, 650 W 168th Street, New York, NY 10032, USA; Corresponding author
Summary: The response of vital organs to different types of nutrition or diet is a fundamental question in physiology. We examined the cardiac response to 4 weeks of high-fat diet in mice, measuring cardiac metabolites and mRNA. Metabolomics showed dramatic differences after a high-fat diet, including increases in several acyl-carnitine species. The RNA-seq data showed changes consistent with adaptations to use more fatty acid as substrate and an increase in the antioxidant protein catalase. Changes in mRNA were correlated with changes in protein level for several highly responsive genes. We also found significant sex differences in both metabolomics and RNA-seq datasets, both at baseline and after high fat diet. This work reveals the response of a vital organ to dietary intervention at both metabolomic and transcriptomic levels, which is a fundamental question in physiology. This work also reveals significant sex differences in cardiac metabolites and gene expression.
