The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle
Faye M Nixon,
Cristina Gutiérrez-Caballero,
Fiona E Hood,
Daniel G Booth,
Ian A Prior,
Stephen J Royle
Affiliations
Faye M Nixon
Division of Biomedical Cell Biology, Warwick Medical School, Coventry, United Kingdom; Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom
Cristina Gutiérrez-Caballero
Division of Biomedical Cell Biology, Warwick Medical School, Coventry, United Kingdom
Fiona E Hood
Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom
Daniel G Booth
Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom
Ian A Prior
Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom
Kinetochore fibers (K-fibers) of the mitotic spindle are force-generating units that power chromosome movement during mitosis. K-fibers are composed of many microtubules that are held together throughout their length. Here, we show, using 3D electron microscopy, that K-fiber microtubules (MTs) are connected by a network of MT connectors. We term this network ‘the mesh’. The K-fiber mesh is made of linked multipolar connectors. Each connector has up to four struts, so that a single connector can link up to four MTs. Molecular manipulation of the mesh by overexpression of TACC3 causes disorganization of the K-fiber MTs. Optimal stabilization of K-fibers by the mesh is required for normal progression through mitosis. We propose that the mesh stabilizes K-fibers by pulling MTs together and thereby maintaining the integrity of the fiber. Our work thus identifies the K-fiber meshwork of linked multipolar connectors as a key integrator and determinant of K-fiber structure and function.
